Constance Ezemba

Constance Ezemba

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Improved Cultural Conditions for Methionine Accumulation in Submerged Cultivation of Bacillus Cereus S8

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Aims: To improve the cultural conditions for enhanced methionine production by Bacillus cereus S8 Study design: Study of the fermentation process in shake flask culture. Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi Azikwe University, Awka, Nigeria between 2011 to 2012. Methodology: The effects of medium/fermenter volume ratio, carbon and nitrogen sources, growth stimulators, vitamins and amino acid on methionine accumulation in the broth culture of Bacillus cereus S8 were investigated. The time course for methionine production was also studied. Results: A 20% medium/fermenter volume ratio improved methionine yield. Glucose and ammonium sulphate at 6.0 and 1.0% respectively stimulated methionine accumulation by Bacillus cereus S8. Yeast extract, peptone, DL-leucine and all vitamins studied enhanced methionine production. A methionine yield of 3.23mg/ml was produced after 96h fermentation and at a pH of 6.90. Conclusion: Improving the cultural conditions of Bacillus cereus S8 in submerged medium stimulated methionine increase.

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Optimization of Fermentation Conditions of Bacillus Thuringiensis Ec1 for Enhanced Methionine Production

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Bacillus thuringiensis EC1, isolated from the fermented oil bean seed, Pentachletra macrophila Benthan, produced a methionine yield of 1.89 mg/ml. The influence of cultural conditions on methionine accumulation by B. thuringiensis EC1 showed that a 20% medium/fermenter volume ratio and a 5% inoculum size increased methionine yield. The carbon of choice was maltose and at 8% level stimulated methionine production. Among the nitrogen sources studied, ammonium sulphate was found to be the best and at 1% concentration produced a methionine yield of 2.56 mg/ml. All growth-promoting substances and their mixtures enhanced methionine accumulation by B. thuringiensis EC1. The effect of Vitamins on methionine production showed that riboflavin and thiamine HCl at 1.0 μg/ml yielded 2.49 mg/ml and 2.80 mg/ml methionine respectively. The influence of bivalent metals on methionine accumulation indicated that Zn2+ at all concentration stimulated methionine production. Mg2+ and Ba2+ at 0.1 μg/ml and 10.0 μg/ml respectively improved methionine yield. Optimizing the cultural conditions of B. thuringiensis EC1 in submerged medium gave a methionine yield of 3.18 mg/ml.

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