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Optimization of Fermentation Conditions of Bacillus Thuringiensis Ec1 for Enhanced Methionine Production

By Constance Ezemba

Summary

Bacillus thuringiensis EC1, isolated from the fermented oil bean seed, Pentachletra macrophila
Benthan, produced a methionine yield of 1.89 mg/ml. The influence of cultural conditions on methionine
accumulation by B. thuringiensis EC1 showed that a 20% medium/fermenter volume ratio
and a 5% inoculum size increased methionine yield. The carbon of choice was maltose and at
8% level stimulated methionine production. Among the nitrogen sources studied, ammonium
sulphate was found to be the best and at 1% concentration produced a methionine yield of 2.56
mg/ml. All growth-promoting substances and their mixtures enhanced methionine accumulation
by B. thuringiensis EC1. The effect of Vitamins on methionine production showed that riboflavin
and thiamine HCl at 1.0 μg/ml yielded 2.49 mg/ml and 2.80 mg/ml methionine respectively. The
influence of bivalent metals on methionine accumulation indicated that Zn2+ at all concentration
stimulated methionine production. Mg2+ and Ba2+ at 0.1 μg/ml and 10.0 μg/ml respectively improved
methionine yield. Optimizing the cultural conditions of B. thuringiensis EC1 in submerged
medium gave a methionine yield of 3.18 mg/ml.
Optimization of Fermentation Conditions of Bacillus Thuringiensis Ec1 for Enhanced Methionine Production
 
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Published: April 30, 2014

Uploaded by: Constance Ezemba

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Constance Ezemba

Constance Ezemba

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