Improved Cultural Conditions for Methionine Accumulation in Submerged Cultivation of Bacillus Cereus S8
By Constance Ezemba
Study design: Study of the fermentation process in shake flask culture.
Place and Duration of Study: Department of Applied Microbiology and Brewing, Nnamdi
Azikwe University, Awka, Nigeria between 2011 to 2012.
Methodology: The effects of medium/fermenter volume ratio, carbon and nitrogen
sources, growth stimulators, vitamins and amino acid on methionine accumulation in the
broth culture of Bacillus cereus S8 were investigated. The time course for methionine
production was also studied.
Results: A 20% medium/fermenter volume ratio improved methionine yield. Glucose and
ammonium sulphate at 6.0 and 1.0% respectively stimulated methionine accumulation by
Bacillus cereus S8. Yeast extract, peptone, DL-leucine and all vitamins studied enhanced
methionine production. A methionine yield of 3.23mg/ml was produced after 96h
fermentation and at a pH of 6.90.
Conclusion: Improving the cultural conditions of Bacillus cereus S8 in submerged
medium stimulated methionine increase.
Published: May 6, 2014
Uploaded by: Constance Ezemba
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