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Antibacterial Screening of the Ethanolic and Aqueous Extract of the Fruit Peel of Persea Americana Mill Against Selected Enteric Bacteria

Antibacterial Screening of the Ethanolic and Aqueous Extract of the Fruit Peel of Persea Americana Mill Against Selected Enteric Bacteria

Uploaded by Amaka Rita Ayeh

The antibacterial sensitivity test showed that the inhibitory effect of the ethanolic extract on E.coli>Salmonella typhi>Proteus vulgaris with mean zones of inhibition of 6.643, 5.71 and 4.57mm respectively while that of the aqueous extract, Salmonella typhi> Proteus vulgaris>E.coli with mean zones of inhibition of 4.57, 0.86 and 0.00mm respectively. The null hypothesis was accepted; ethanoic extract and aqueous extract had inhibitory activity against the three organisms at 95% confidence interval. In comparing the activity of the two extracts, there is a significant difference between the activity of the aqueous extract and ethanolic extract against these bacteria at 95% confidence level in this order of concentration 62.5>1000 and 750mg/ml>negative control i.e. there is no significant difference in the activity between the two extracts at 500, 250 and125mg/ml. This implies that at 500, 250 and 125mg/ml there was a significant antibacterial activity against the clinical isolates irrespective of the extract used. From the mean plots, it can be deduced that the activity of the treatments is not dependent on concentration as the measure of susceptibility did not follow an increase or decrease in concentration. With both extract, S.typhi was best inhibited at 500mg/ml. E.coli was best inhibited at 1000mg/ml for ethanolic extract but not inhibited by aqueous, it cannot therefore be concluded that the aqueous extract has no effect on E.coli because a higher concentration of the aqueous extract might inhibit E.coli. Proteus vulgaris had best inhibition at 500mg/ml for ethanol extract and 500 and 250mg/ml for aqueous extract. It can be said that both extract would work best at 500mg/ml of treatment for the bacteria especially P.vulgaris and Salmonella typhi, though a higher concentration is suspected to have activity against E.coli.

 
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